Cells communicate via exosomes, nano-sized packages of information released by cells and absorbed by other cells in a functionally relevant manner. These packages of information contain a variety of proteins, genetic material and other cargo which have the ability to induce changes in recipient cells. Under certain conditions, exosomes produced by stem cells can be manipulated to carry different cargos. A wide variety of therapeutic molecules can be loaded into exosomes to protect the active molecule from breakdown and to target a specific area of the body. We have therefore developed a technology by which a permanent stem cell line can be cultured to produce specific therapeutic agents that can be harvested at a commercially relevant scale. The ability to produce commercially valuable therapeutic products from stem cell-derived exosomes demands a standardised stem cell producer line appropriately sourced and isolated, manufactured to GMP, grown in serum-free conditions and (ideally) already having demonstrated patient safety. In the stem cell field, our CTX cell line uniquely meets all these conditions.
Summary of exosomes for therapy/ delivery vehicle:
- Exosomes can be loaded to carry a variety of therapeutic agents for delivery to specific tissues within the body.
- Stem cell-derived exosomes have generally been found to be less immunogenic than cells. Thus, “off the shelf” products may readily be developed using exosomes with negligible immunogenicity.
- Exosomes can be stored without cryo-preservatives at -20°C for 6 months with no loss in their biochemical activities.
- An important feature of exosomes is encapsulation. Protecting their contents from degradation, thereby, preventing the active ingredients from being rapidly degraded.
- The durability of exosomes in culture permits large quantities of exosomes to be harvested and collected from cell culture medium.
- By changing the exosome producer cell type, you can alter the region to which the payload is delivered.
ReNeuron has signed a collaboration agreement with a US-based biopharmaceutical company to explore the use of our exosome technology as a delivery vehicle for synthetic oligonucleotides used in gene therapy and is also in active discussions with other third parties regarding further potential collaborations.
Please click below to watch an animation which illustrates how exosomes function: https://vimeo.com/118608839