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ReNeuron's Myc immortalised clonal neural stem cell lines demonstrate a variety of neural phenotypes in culture
Myc immortalised cortical cell line (CTXOA32) in the presence of growth factors bFGF and EGF. Cultures are predominantly GFAP staining astrocytes (red) with a few ß III-tubulin staining neurones (green). Counterstaining of cell nuclei with Hoechst dye.
Myc immortalised cortical cell line (CTXOA32) differentiating in the absence of growth factor for 7 days. Cultures are a mixed population of GFAP staining astrocytes (red) and ßIII-tubulin staining neurones (green). Counterstaining of cell nuclei with Hoechst dye.
MycERTam (tamoxifen regulated myc) immortalised cortical cell line (CTXOJ10) in the absence of growth factors bFGF and EGF for 7 days. Staining for beta III tubulin (green) with counterstaining of cell nuclei with Hoechst dye (blue) shows an abundance of neuronal differentiation.
MycERTam (tamoxifen regulated myc) immortalised cortical cell line (CTXOJ10) in the absence of growth factors bFGF and EGF for 7 days. Staining for GFAP (red) with counterstaining of cell nuclei with Hoechst dye (blue) shows a high level of astrocytic differentiation.
ReNcellTM lines: functional neural networks with different neuronal phenotypes in culture
Differentiating ReNcell VM showing developing neurones stained for ßIII-tubulin (green) and a backdrop of GFAP staining astrocytes (red). Nucelei are counterstained with Hoechst (blue).
Differentiating ReNcell CX showing developing neurones stained for ßIII-tubulin (green) and a backdrop of GFAP staining astrocytes (red). Nucelei are counterstained with Hoechst (blue).
TH positive neurones (pink) in differentiated, early passage ReNcell VM ßIII-tubulin staining neurones (green) with Hoechst counterstaining (blue).
Staining for TH neurones only.
Patch clamp analysis of Differentiated ReNcell CX cells showing one population of cells with low resting membrae potential (-20mV) and another population at -80mV.
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