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ReNeuron's immortalised clonal neural stem cell lines demonstrate a variety of neural phenotypes in culture
Immortalised cortical cell line (CTXOA32) in the presence of growth factors bFGF and EGF. Cultures are predominantly GFAP staining astrocytes (red) with a few ß III-tubulin staining neurons (green). Counterstaining of cell nuclei with Hoechst dye.
Immortalised cortical cell line (CTXOA32) differentiating in the absence of growth factor for 7 days. Cultures are a mixed population of GFAP staining astrocytes (red) and ßIII-tubulin staining neurons (green). Counterstaining of cell nuclei with Hoechst dye.
Immortalised cortical cell line (CTXOJ10) in the absence of growth factors bFGF and EGF for 7 days. Staining for beta III tubulin (green) with counterstaining of cell nuclei with Hoechst dye (blue) shows an abundance of neuronal differentiation.
Immortalised cortical cell line (CTXOJ10) in the absence of growth factors bFGF and EGF for 7 days. Staining for GFAP (red) with counterstaining of cell nuclei with Hoechst dye (blue) shows a high level of astrocytic differentiation.
ReNcell® lines: functional neural networks with different neuronal phenotypes in culture
Differentiating ReNcell®VM showing developing neurons stained for ßIII-tubulin (green) and a backdrop of GFAP staining astrocytes (red). Nuclei are counterstained with Hoechst (blue).
Differentiating ReNcell®CX showing developing neurons stained for ßIII-tubulin (green) and a backdrop of GFAP staining astrocytes (red). Nuclei are counterstained with Hoechst (blue).
TH positive neurons (pink) in differentiated, early passage ReNcell VM ßIII-tubulin staining neurons (green) with Hoechst counterstaining (blue).
Differentiated, early passage ReNcell®VM showing TH-positive neurons (pink),
Patch clamp analysis of Differentiated ReNcell®CX cells showing one population of cells with low resting membrae potential (-20mV) and another population at -80mV.
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